Journal: OncoTargets and therapy

Article Title: Identification of 1,2,4-Oxadiazoles-Based Novel EGFR Inhibitors: Molecular Dynamics Simulation-Guided Identification and in vitro ADME Studies

doi: 10.2147/OTT.S357765

Figure Lengend Snippet: IC 50 determination from EGFR kinase activity assay in ( A ) EFGR WT and ( B ) EGFR T790M ; Data represented as mean ± standard deviation; N = 3, GraphPad Prism software was used to calculate IC 50 values.

Article Snippet: MCF7 and MRC5 cell lines were used from ATCC; LDH Cytotoxicity Assay Kit, Item No. 601170 was used from Cayman Chemical; EGFR Kinase assay kit, Catalogues no. 40321 and 40323 were used from BPS Bioscience; Caco-2 (human colon carcinoma) cell line, borrowed from NCCS, Pune; which was initially procured from American Type Culture Collection (ATCC).

Techniques: Kinase Assay, Standard Deviation, Software

Journal: RSC Advances

Article Title: A new class of anti-proliferative activity and apoptotic inducer with molecular docking studies for a novel of 1,3-dithiolo[4,5- b ]quinoxaline derivatives hybrid with a sulfonamide moiety †

doi: 10.1039/d3ra01635h

Figure Lengend Snippet: Level of EGFR Wt , EGFR (L858R) , and VEGFR2 following the treatment of MCF-7 cells with the IC 50 dose of the most active derivative 12 and positive controls

Article Snippet: Additionally, the in vitro EGFR wt , EGFR L858R , and VEGFR for the most active derivative 12 were performed using the BPS-Bioscience EGFR Kinase Assay Kit Catalog #40321, EGFR(L858R) Kinase Assay Kit Catalog #40324, and VEGFR-2(KDR) Kinase Assay Kit Catalog #40325, respectively, according to the manufacturer's instructions.

Techniques: Activity Assay